Cloning and/or sequencing vector

ABSTRACT

A cloning and/or sequencing vector (1) comprises a nucleotide promoter sequence (3) and a nucleotide sequence (4) coding for a fusion protein active as a poison, which nucleotide sequence (4) is obtained by fusion of a coding nucleotide sequence (5) containing several unique cloning sites with a nucleotide sequence (6) coding for a poison protein, wherein the nucleotide sequences (3) and (4) are incorporated in an autonomous replication vector (2).

This application is a 371 of PCT/BE93/00051 filed Aug. 2, 1993.

SUBJECT-MATTER OF THE INVENTION

The invention relates to a cloning and/or sequencing vector whichenables recombinant clones to be selected directly.

The invention also relates to the procaryotic cell which is transformedby this vector and to the procaryotic host cell for this vector, as wellas to the use of this cloning and sequencing vector for selecting andsequencing recombinant clones.

STATE OF THE ART AND TECHNOLOGICAL BACKGROUND UNDERLYING THE INVENTION

Phage (the M13 series) and plasmid (the pUC series) cloning vectors,containing numerous unique cloning sites, were constructed by Messing etal (P.N.A.S. USA, 79, pp. 3642-3646 (1977), by Norrander et al (Gene,26, pp. 101-106 (1983) and Yanisch-Perron et al (Gene, 33pp. 103 to 119)(1985)).

The multiple cloning sites (MCS-multiple cloning sites) of these vectorsare located in the coding sequence of the LacZ gene.

Discrimination between the transformed cells which harbor a recombinantvector and the cells which harbor a non-recombinant vector is achievedusing the "blue screen" technique described by Gronenborn and Messing(Methylation of single-stranded DNA in vitro introduces new restrictionendonuclease cleavages sites, Nature, 272, pp. 375-377 (1978)).

However, this "blue screen" technique suffers from the disadvantage ofusing a screening procedure (discrimination) rather than a procedure forselecting the clones.

Discrimination by screening is based on identifying a clone within apopulation of clones on the basis of a characteristic (color) whichdifferentiates it. Selection has no need of this characteristic, sinceit is only recombinant clones which are isolated by this method.

The screening procedure is based on the color of the recombinant clones(white color) and of the non-recombinant clones (blue color). This coloris based on inactivation of the marker beta-galactosidase, preventingcleavage of X-gal (5-bromo-4-chloro-3-indolyl β-galactoside). The cellcolonies harboring a non-recombinant vector produce a functionalbeta-galacto-sidase and, by hydrolyzing the X-gal substrate, produce ablue coloration. In general, the insertion of a DNA fragment into theβ-galactosidase gene prevents cleavage of the X-gal. For this reason,the cells harboring a recombinant vector have a white color.

Moreover, this complex procedure requires the use of the substrate X-galwhich is a product which is very expensive, unstable and awkward to use.

On the other hand, various cloning vectors permitting direct selection(positive selection) of recombinant strains have been described in thescientific literature.

Pierce et al (Proc. Natl. Acad. Sci., vol 89. No. 6, 1992, pp.2056-2060) describe a vector which comprises the lethal gene sac B fromBacillus amylolique-faciens, integrated into a plasmid derived from thebacteriophage P1 and under the control of a specific E. coli promoter.

The promoter of this vector includes a region having several specificcloning sites (cleavage site for a restriction enzyme).

Since the gene sac B encodes levan sucrase, which catalyzes thehydrolysis of sucrose into products which are toxic for E. coli, directselection of the mutants which incorporate a recombinant plasmid iseffected on a culture medium containing sucrose. Since the levan sucraseis toxic, even in the absence of sucrose, it is essential, consequently,to repress its synthesis if one wishes to obtain a large number ofplasmid copies in the bacterial cytoplasm.

However, it is difficult, if not impossible, to repress the cytotoxicgene completely, particularly if a large number of copies of the vectorare required.

Therefore, the impossibility of repressing the cytotoxic gene leads, inphases of producing the plasmid, to the death of the cell and, as aconsequence, to selective pressure towards mutated strains(characterized by an inactive lethal gene).

In this case, in order to ensure that the enzyme encoded by the sac Bgene does not kill the host cell, it is necessary to incorporate a CIrepressor, which regulates the expression of this gene, into the cloningvector.

Furthermore, since sucrose is often incorporated into bacterial culturemedia, it will be essential to prepare media which are totally free ofsucrose in order to carry out these manipulations.

Henrich et al (Gene, vol 42, No. 3, 1986, pp. 345-349) describe a vectorwhich includes the E gene from the bacteriophage φX174, the said E genebeing incorporated into the plasmid pUH84 under the control of the Lacpromoter.

In this case, the E gene includes six unique restriction sites (locatedover the whole of the E gene sequence) and encodes gpE, which causeslysis of the E.coli cell. In this case, positive selection is effectedwhen a foreign recombinant gene has been inserted into one of therestriction sites.

However, this insertion of a foreign gene into a restriction sitelocated in the sequence of the E gene, encoding gpE, makes it moredifficult to sequence the foreign gene and/or amplify it by PCR since,in this case, portions of useless sequences belonging to the E geneencoding gpE are also sequenced, amplified and characterized.

Kuhn et al (Gene, vol 42, No. 3, 1986, pp. 253-263) describe a vectorwhich includes a large gene encoding a restriction enzyme which kills bycleaving the genome of the bacterium, the said gene being incorporatedinto the plasmid pKG2 under the control of the LacUV5 promoter.

The cloning vectors of the state of the art suffer from the disadvantageof having to be maintained in a host strain which includes the LacI_(q)repressor in episomal form, or the CI repressor, in order to inactivatethe promoter and prevent expression of the killer gene, thus leading tothe death of the host strain.

In addition, if it is desired to use this strain to produce a largenumber of copies of the cloning vectors, the repressor will not beadequate for preventing either a selective pressure which modifies thecytotoxic activity of the vector or a "genetic leakage", that is to sayexpression of certain copies of the vector and death of the host strain.

Consequently, none of the documents of the state of the art describes acloning vector which can incorporate large nucleotide fragments, whichis easy to manipulate and which can be produced by a microorganism on anindustrial scale; that is to say, which can be produced in a largenumber of copies by a microorganism without bringing about the death ofthe latter.

OBJECTS OF THE INVENTION

The present invention aims to supply a novel cloning and/or sequencingvector, and also its host strain, which are simple and relativelyinexpensive to construct and produce, and which enable recombinantclones to be selected directly, without suffering from the disadvantagesof the abovementioned state of the art.

A particular object of the present invention is to obtain a vector whichpermits specific and certain selection of the recombinant clones.

Another object of the present invention is directed towards obtaining avector which permits the sequencing, amplification and/orcharacterization, using the same primer, of any foreign DNA fragment(whatever its size) in the recombinant clones.

An additional object of the present invention is directed towardsobtaining a vector which also permits simple extraction of this foreignDNA fragment from the recombinant clone.

A final object of the present invention is directed towards obtaining ahost strain for the said vector which allows a large number of copies ofthe said vector to be produced without bringing about selection pressurewhich modifies the cytotoxic activity of the said vector or causing thedeath of the host strain.

CHARACTERISTIC ELEMENTS OF THE INVENTION

The invention relates to a novel cloning and/or sequencing vector whichincludes, incorporated into an autonomously replicating vector, at leastone promoter nucleotide sequence and at least one nucleotide sequenceencoding a fusion protein which is active as a poison, the saidnucleotide sequence being obtained by fusing a coding nucleotidesequence which includes several unique cloning sites and a nucleotidesequence which encodes a protein poison.

Preferably, the autonomously replicating vector is a recombinant virusor a recombinant plasmid such as a pUC plasmid.

The promoter nucleotide sequence can comprise any promoter, whichpermits expression of the nucleotide sequence encoding a fusion proteinwhich is active as a poison.

Preferably, this promoter nucleotide sequence consists of the Lac operonpromoter.

According to one preferred embodiment of the invention, the uniquecloning sites (MCS) of the nucleotide sequence which is fused to thenucleotide sequence which encodes the protein poison are absent from theremainder of the nucleotide sequence of the vector according to theinvention.

Advantageously, the nucleotide sequence of the gene which encodes theprotein poison comprises all or part of the nucleotide sequence of thewild-type gene which encodes the protein CcdB.

Preferably, the nucleotide sequence of the gene which encodes theprotein poison lacks the cleavage site for the restriction enzyme SmaI.

Another aspect of the invention relates to a procaryotic cell which istransformed with the cloning vector according to the invention.

The invention also relates to a procaryotic host cell for the vectoraccording to the invention which possesses a chromosomal I^(q) and anelevated transformation efficiency, and which possesses a mutationconferring resistance to the poison activity of the fusion protein,and/or which possesses a gene encoding a protein which is an antipoisonto the fusion protein.

Preferably, the procaryotic host cell for the vector according to theinvention possesses a mutation in the gene encoding subunit A, or in thegene encoding subunit B, of the gyrase, and conferring resistance to thefusion protein, and/or a gene which encodes the protein CcdA which is anantipoison to the fusion protein.

Preferentially, the procaryotic cell is an Escherichia coli cell whichpossesses a mutation which is responsible for replacing arginine 462with a cysteine in the amino acid sequence of the GyrA polypeptide ofthe gyrase, thereby conferring resistance to the fusion protein.

Preferably, this procaryotic host cell also possesses the LacI^(q)mutation.

The present invention also relates to fragments of the vector accordingto the invention, in particular primers for sequencing and/or amplifying(for example by PCR) the foreign nucleotide fragments inserted into thevector according to the invention.

Preferably, these primers consist of sequences of from 10 to 30nucleotides which hybridize to nucleotide sequences which are situatedon either side of the nucleotide sequence of the vector according to theinvention which contains several unique cloning sites.

A final aspect of the invention relates to the use of the vectoraccording to the invention for selecting and sequencing recombinantclones.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a diagrammatic representation of a cloning vector according tothe present invention.

DESCRIPTION OF A PREFERRED EMBODIMENT OF THE INVENTION

According to the invention, the cloning and/or sequencing vector 1includes, incorporated into an autonomously replicating vector 2, atleast one promoter nucleotide sequence 3 and at least one nucleotidesequence 4 which encodes a fusion protein which is active as a poison,the said nucleotide sequence 4 being obtained by fusing a codingnucleotide sequence 5 (or polylinker) which encompasses several(multiple) unique cloning sites (MCS), and a nucleotide sequence (6)which encodes a protein poison.

An autonomously replicating vector 2 is understood to mean anynucleotide construct, such as a virus or a plasmid (preferably arecombinant plasmid of the PUC series), which is capable of beingintroduced into a microorganism, of recombining therein and/or ofreplicating therein.

FIG. 1 shows a diagrammatic representation of a cloning vector accordingto the present invention, which vector is constructed from a plasmid ofthe pUC series (pUC18 and pUC19), which is described by Norrander et al(Construction of improved M13 vectors usingoligodeoxinucleotide-directed mutagenesis, Gene, 26, pp. 101-106 (1983))and by Yanisch-Perron et al (Improved M13 phage cloning vectors and hoststrains: nucleotide sequences of the M13mp18 and pUC19 vectors, Gene,33, pp. 103-119 (1985)).

A coding nucleotide sequence 5 encompassing several (multiple) uniquecloning sites (MCS) is understood to mean a short coding sequence (orpolylinker) which comprises several cleavage sites for restrictionenzymes.

The advantage of having a polylinker in the vector according to theinvention is that different cloning sites are located on a single shortsequence, thereby permitting:

rapid sequencing and amplification, using the same primers, of any DNAfragment which is inserted into this vector.

rapid extraction of the cloned fragment, facilitated by the proximity ofthe restriction sites. Thus, in contrast to the state of the art, thisproximity avoids sequencing, amplifying and characterizing uselessfragments from other sequences of the vector according to the invention.

Nucleotide sequence 6 encoding a protein poison is understood to meanany (wild-type) nucleotide structure encoding a protein which displaysan activity which is naturally poisonous and specific for one or morevital functions of a host cell.

A protein poison is also characterized by the existence of an antidoteor antipoison, such as the proteins CcdB and CcdA, the protein Kid andits antagonist Kis, the protein PemK and its antagonist PemI, theprotein Doc and its antagonist Phd, the protein HoK and its antagonistSok, and other poison molecules which are, or are not, of plasmidorigin.

In this case, the nucleotide sequence 6 encoding a protein poisonconsists of the wild-type gene CcdB, which encodes the protein CcdB(control of cell death), obtained from the ccd locus of the F plasmid(SEQ ID NO:1 and SEQ ID NO:4.

The ccd locus of the F plasmid comprises the two wild-type genes ccdAand ccdB, also termed H and G, or letA and letD, which respectivelyencode proteins of 72 and 101 amino acids (Bex et al, Mini-F encodedproteins; identification of a new 10.5 kilodalton species. EMBO J.2,1853-1861 (1983); Miki et al, Control of cell division by sex factor Fin Escherichia coli. I. The 42.84-43.6 F segment couples cell divisionof the host bacteria with replication of plasmid DNA, J. Mol. Bio.,procaryotic cell which is transformed with the cloning 174, 605-625,(1984)).

In Escherichia coli, the CcdB protein of the F plasmid is a cytotoxinwhose lethal activity is counteracted by the protein CcdA (Karoui et al,Ham22, a mini-F mutation which is lethal to host cell and promotesrecA-dependent induction of lambdoid prophage. EMBO J.2, 1863-1868(1983); Ogura and Hiraga Mini-F plasmid gene that couple host celldivision to plasmid proliferation, Proc. Natl. Acad. Sci. USA, 80,4784-4788 (1983); Miki et al, Control of cell division by sex factor Fin Escherichia coli. Identification of genes for inhibitor protein andtrigger protein on the 42.84-43.6F segment, J. Mol. Biol. 174, 627-646(1984b)).

The molecular mechanism by which protein CcdB exerts its lethal activityhas been elucidated; protein CcdB is poisonous to DNA topoisomerase II.

The type II DNA topoisomerases are essential and ubiquitous enzymeswhich alter the topology of the DNA by transiently introducing adouble-stranded break into the DNA. During the stage ofbreak-religation, topoisomerase II forms an intermediate complex withits DNA substrate in which the enzyme is attached covalently to the 5'end of the cleaved DNA. This transitory intermediate, in whichtopoisomerase II is linked covalently to the DNA, has been termed the"cleavable complex" (Wang, DNA topoisomerases. Annu. Rev. Biochem. 54,665-97, 1985; Maxwell & Gellert, Mechanistic aspects of DNAtopoisomerases. Advan. Protein Chem. 38, 69-107, 1986; Liu, DNAtopoisomerase poisons as antitumor drugs, Annu. Rev. Biochem. 58.351-375, 1989).

Both in eucaryotes and in procaryotes, the cleavable topoisomeraseII-DNA complex is the target of powerful therapeutic agents, includingthe antibiotics of the "quinolone" family, which act on the gyrase(bacterial topoisomerase II), and anticancer agents (acridines andepipodophyllotoxins), which act on the mammalian topoisomerase II. Thetherapeutic efficacy of the topoisomerase poisons is correlated withtheir ability to stabilize the cleavable complex.

DNA topoisomerase II is an essential enzyme in all living entities andis very conserved in the evolution of the species. The CcdB protein thusdisplays an activity which is potentially cytotoxic for a wide varietyof procaryotic species.

The small size of the wild-type ccdB gene allows it to be inserted intoplasmids without increasing their size excessively and consequentlyallows large fragments of foreign DNA to be included therein.Furthermore, given its small size, the wild-type ccdB gene of the Fplasmid contains very few restriction sites; it is, therefore, simplerto preserve the uniqueness of the multiple cloning sites (MCS) which areadded to it.

Unexpectedly, the inventors observed that the in-phase fusion of thenucleotide sequence 6, encoding protein CcdB, with the coding nucleotidesequence (polylinker 5), comprising several (multiple) unique cloningsites (MCS), gave a nucleotide sequence 4 which encodes a fusion proteinwhich is active as a poison and which makes it possible, as aconsequence, to produce vectors for the direct selection of recombinantplasmids (killer selection).

The plasmids which have been obtained allow doubly digested restrictionfragments to be cloned in both orientations with respect to the lacpromoter. Insertion of a restriction fragment into one of the uniquecloning sites interrupts the genetic information of the gene fusion,leading to the synthesis of a gene fusion product which is notfunctional. Insertional inactivation of the gene fusion ought always totake place when a termination codon is introduced or when a change ismade in the reading frame.

The cells which harbor an intact cloning vector of this nature produce apoisonous fusion protein which is functional, and die.

Insertion of a foreign DNA fragment into one of the unique cloning sitesof the gene fusion interferes with production of the poison.

The cells which harbor a recombinant vector will be viable while cellswhich harbor an intact vector will not be viable. This killer selection,by simple culture on a solid medium, makes it possible to eliminatecells which harbor a non-recombinant vector (non-viable clones) and toselect recombinant clones (viable clones).

EXAMPLE I

Construction of the plasmid pKIL19

The ccdb gene was amplified by PCR using, as DNA template, the plasmidpULB2208 (Bernard and Couturier, The 41 carboxy-terminal residues of theminiF plasmid CcdA protein are sufficient to antagonize the killeractivity of the CcdB protein, Mol. Gen. Genet. 226, 297-304 (1991) aswell as synthetic oligonucleotides.

The synthetic oligonucleotide sequences were selected in such a way asto create an EcoRI restriction site on either side of the wild-type ccdBgene in order to be able to reclone this gene in frame with the codonsof the MCS19 multiple cloning site and to eliminate the initiation codonof the native ccdb gene. The DNA resulting from the PCR reaction wasdigested with the enzyme EcoRI and cloned into the EcoRI site of theplasmid pUC19. The resulting plasmid, in which the EcoRI fragment wasintegrated in the orientation which permitted the ccdB gene, providedwith the additional codons corresponding to the MCS19 multiple cloningsites, to be read from the Lac promoter, was termed pKIL2. Plasmid pKIL2is lethal for a wild-type bacterium (CcdBS sensitive).

pKIL2 also possesses two SmaI sites, one in the multiple cloning sitesand the other in the central region of the ccdB gene. The latter waseliminated by site-directed mutagenesis. The resulting plasmid pKIL3,having a unique SmaI site, still has two EcoRI sites. The EcoRI sitedownstream of the ccdB gene was eliminated by filling in its cohesiveends.

The resulting plasmid, pKIL19 (SEQ ID NO:2 and SEQ ID NO:5), thuspossesses a unique EcoRI restriction site within sequence 5, whichencompasses the multiple cloning site.

EXAMPLE II

Construction of the plasmid pKIL18

The ccdB gene was amplified by PCR using, as DNA template, plasmidpKIL19 as well as synthetic oligonucleotides. The sequences of thesynthetic oligonucleotides were selected in such a way as to create aHindIII site on either side of the ccdb gene in order to be able toreclone this gene in frame with the codons of the MCS18 multiple cloningsites. The DNA resulting from the PCR reaction was digested by theenzyme HindIII and cloned into the HindIII site of the plasmid pUC18.The resulting plasmid, in which the HindIII fragment was integrated inthe orientation which permitted the ccdB gene, provided with theadditional codons corresponding to the MCS18 multiple cloning sites, tobe read from the Lac promoter, was termed pKIL4. Plasmid pKIL4 is lethalfor a CcdB^(s) -sensitive bacterium.

The HindIII site downstream of the ccdB gene was eliminated by fillingin its cohesive ends. The resultant plasmid, pKIL18 (SEQ ID NO:3 and SEQID NO:6) possesses a unique HindIII restriction site as well as a uniqueSmaI site (since constructed from pKIL19).

EXAMPLE III

Construction of the strains CcdB^(r) and CcdB^(s)

In order to be able to maintain plasmids pKIL18 and pKIL19 within abacterium, the latter has to be resistant to the lethal effect of thefusion protein which is active as a poison. Unexpectedly, thechromosomal mutation gyrA462 confers on the strains total resistance tothe poisonous effect of the fusion protein.

Moreover, since plasmids pKIL18 and pKIL19 derive directly from plasmidspUC18 and pUC19 and express the ccdB genes from the Lac promoter, it ispreferable to maintain these plasmids in a LacI^(q) strain. Thus, while,in our case, continuous overexpression of these genes does not exert aselection pressure in favor of certain mutations, the LacI^(q) strainallows expression from the Lac promoter to be reduced and conserves thebacterial machinery, thereby guaranteeing a rapid generation time(increased production of the vector by the strain).

The strain D1210 (Sadler et al Gene 8, pp. 279-300 (1980)), derived fromthe strain HB101 LacI^(q), LacY⁺ (Maniatis et al Molecular CloningLaboratories Man. Cold Spring Harbor Laboratory New York), andcharacterized by a chromosomal I^(q) and increased transformationefficiency, was transformed with the plasmid pCOS2.1. This plasmid,which confers resistance to kanamycin, carries the recA gene fromErwinia chrysanthemi 3665 and allows recombination in E, coli. A lysateof P1 phage was prepared on a CcdB^(R) gyrA462, zei298::Tn10 strain andused to infect the strain D1210/pCOS2.1. The transductants which wereresistant to tetracycline were selected and tested for their resistanceor sensitivity to the CcdB protein. One of the CcdB^(R) transductantswas then cured of plasmid pCOS2.1 and termed KIB22.

Strain KIB22 constitutes an ideal host strain for plasmids pKIL18 andpKIL19 while strain D1210 constitutes the ideal host for selectingrecombinant plasmids.

Thus, strain KIB22 advantageously possesses an elevated efficiency ofDNA extraction (comparable to the yield of the pUC plasmids) and,unexpectedly, resistance to the fusion protein which is encoded bypKIL18 and pKIL19.

Consequently, it is possible to use this microorganism to produce thecloning vector according to the invention on an industrial scale innumerous copies without causing the death of the said microorganism.

The selection is carried out simply by spreading the bacteria on amedium containing IPTG (Isopropyl-Beta-D-thiogalactopyranoside) as wellas ampicillin.

Strain KIB22 was deposited with the Laboratorium voorMicrobiologie-Bacterienverzameling (LMG) MicrobiologicalLaboratory--Bacterial Collection! of the Belgian Coordinated Collectionsof Microorganisms (BCCM) under No. LMG P-12601.

The cloning vector pKIL19 was deposited with the Laboratorium voorMoleculaire Biologie-Plasmiden Collectie (LMBP) Molecular BiologicalLaboratory--Plasmid Collection! of the Belgian Coordinated Collectionsof Microorganisms (BCCM) under the No. LMBP 2781.

These depositions were made in accordance with the provisions of theBudapest Treaty regarding the International Recognition of theDeposition of Microorganisms.

    __________________________________________________________________________    #             SEQUENCE LISTING    - (1) GENERAL INFORMATION:    -    (iii) NUMBER OF SEQUENCES: 6    - (2) INFORMATION FOR SEQ ID NO:1:    -      (i) SEQUENCE CHARACTERISTICS:    #pairs    (A) LENGTH: 306 base              (B) TYPE: nucleic acid              (C) STRANDEDNESS: single              (D) TOPOLOGY: both    -     (ii) MOLECULE TYPE: Genomic DNA    -    (iii) HYPOTHETICAL: NO    -     (iv) ANTI-SENSE: NO    -      (v) FRAGMENT TYPE:    -     (vi) ORIGINAL SOURCE:    #ccdB gene of plasmid FL ISOLATE:    -     (ix) FEATURE:              (A) NAME/KEY: Coding Se - #quence              (B) LOCATION: 1...303              (D) OTHER INFORMATION:    -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:    -   ATG CAG TTT AAG GTT TAC ACC TAT AAA A - #GA GAG AGC CGT TAT CGT CTG      48      Met Gln Phe Lys Val Tyr Thr Tyr Lys A - #rg Glu Ser Arg Tyr Arg Leu    #15    -   TTT GTG GAT GTA CAG AGT GAT ATT ATT G - #AC ACG CCC GGG CGA CGG ATG      96      Phe Val Asp Val Gln Ser Asp Ile Ile A - #sp Thr Pro Gly Arg Arg Met    #              30    -   GTG ATC CCC CTG GCC AGT GCA CGT CTG C - #TG TCA GAT AAA GTC TCC CGT     144      Val Ile Pro Leu Ala Ser Ala Arg Leu L - #eu Ser Asp Lys Val Ser Arg    #          45    -   GAA CTT TAC CCG GTG GTG CAT ATC GGG G - #AT GAA AGC TGG CGC ATG ATG     192      Glu Leu Tyr Pro Val Val His Ile Gly A - #sp Glu Ser Trp Arg Met Met    #      60    -   ACC ACC GAT ATG GGC AGT GTG CCG GTC T - #CC GTT ATC GGG GAA GAA GTG     240      Thr Thr Asp Met Gly Ser Val Pro Val S - #er Val Ile Gly Glu Glu Val    #  80    -   GCT GAT CTC AGC CAC CGC GAA AAT GAC A - #TC AAA AAC GCC ATT AAC CTG     288      Ala Asp Leu Ser His Arg Glu Asn Asp I - #le Lys Asn Ala Ile Asn Leu    #95    # 306               ATA TAA      Met Phe Trp Gly Ile                  100    - (2) INFORMATION FOR SEQ ID NO:2:    -      (i) SEQUENCE CHARACTERISTICS:    #pairs    (A) LENGTH: 420 base              (B) TYPE: nucleic acid              (C) STRANDEDNESS: single              (D) TOPOLOGY: both    - 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#al Ile Pro Leu Ala Ser Ala    #      60    -   CGT CTG CTG TCA GAT AAA GTC TCC CGT G - #AA CTT TAC CCG GTG GTG CAT     240      Arg Leu Leu Ser Asp Lys Val Ser Arg G - #lu Leu Tyr Pro Val Val His    #  80    -   ATC GGG GAT GAA AGC TGG CGC ATG ATG A - #CC ACC GAT ATG GCC AGT GTG     288      Ile Gly Asp Glu Ser Trp Arg Met Met T - #hr Thr Asp Met Ala Ser Val    #95    -   CCG GTC TCC GTT ATC GGG GAA GAA GTG G - #CT GAT CTC AGC CAC CGC GAA     336      Pro Val Ser Val Ile Gly Glu Glu Val A - #la Asp Leu Ser His Arg Glu    #             110    -   AAT GAC ATC AAA AAC GCC ATT AAC CTG A - #TG TTC TGG GGA ATA TAAATGTCAG     388      Asn Asp Ile Lys Asn Ala Ile Asn Leu M - #et Phe Trp Gly Ile    #         125    #         420      AAGCTA GCTTGGCACT GG    - (2) INFORMATION FOR SEQ ID NO:3:    -      (i) SEQUENCE CHARACTERISTICS:    #pairs    (A) LENGTH: 416 base              (B) TYPE: nucleic acid              (C) STRANDEDNESS: single              (D) TOPOLOGY: both    -     (ii) MOLECULE TYPE: Genomic DNA    -    (iii) HYPOTHETICAL: NO    -     (iv) ANTI-SENSE: NO    -      (v) FRAGMENT TYPE:    -     (vi) ORIGINAL SOURCE:    #ccdB gene of plasmid pKIL 19ATE:    -     (ix) FEATURE:              (A) NAME/KEY: Coding Se - #quence              (B) LOCATION: 1...378              (D) OTHER INFORMATION:    -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:    -   ATG ACC ATG ATT ACG CCA AGC TTG CAT G - #CC TGC AGG TCG ACT CTA GAG      48      Met Thr Met Ile Thr Pro Ser Leu His A - #la Cys Arg Ser Thr Leu Glu    #15    -   GAT CCC CGG GTA CCG AGC TCG AAT TCA T - #TG CAG TTT AAG GTT TAC ACC      96      Asp Pro Arg Val Pro Ser Ser Asn Ser L - #eu Gln Phe Lys Val Tyr Thr    #              30    -   TAT AAA AGA GAG AGC CGT TAT CGT CTG T - #TT GTG GAT GTA CAG AGT GAT     144      Tyr Lys Arg Glu Ser Arg Tyr Arg Leu P - #he Val Asp Val Gln Ser Asp    #          45    -   ATT ATT GAC ACG CCG GGG CGA CGG ATG G - #TG ATC CCC CTG GCC AGT GCA     192      Ile Ile Asp Thr Pro Gly Arg Arg Met V - #al Ile Pro Leu Ala Ser Ala    #      60    -   CGT CTG CTG TCA GAT AAA GTC TCC CGT G - #AA CTT TAC CCG GTG GTG CAT     240      Arg Leu Leu Ser Asp Lys Val Ser Arg G - #lu Leu Tyr Pro Val Val His    #  80    -   ATC GGG GAT GAA AGC TGG CGC ATG ATG A - #CC ACC GAT ATG GCC AGT GTG     288      Ile Gly Asp Glu Ser Trp Arg Met Met T - #hr Thr Asp Met Ala Ser Val    #95    -   CCG GTC TCC GTT ATC GGG GAA GAA GTG G - #CT GAT CTC AGC CAC CGC GAA     336      Pro Val Ser Val Ile Gly Glu Glu Val A - #la Asp Leu Ser His Arg Glu    #             110    -   AAT GAC ATC AAA AAC GCC ATT AAC CTG A - #TG TTC TGG GGA ATA TAAATGTCAG     388      Asn Asp Ile Lys Asn Ala Ile Asn Leu M - #et Phe Trp Gly Ile    #         125    #            416   GAATTA ATTCAGTG    - (2) INFORMATION FOR SEQ ID NO:4:    -      (i) SEQUENCE CHARACTERISTICS:    #acids    (A) LENGTH: 101 amino              (B) TYPE: amino acids              (C) STRANDEDNESS: single              (D) TOPOLOGY: linear    -     (ii) MOLECULE TYPE: protein    -    (iii) HYPOTHETICAL: NO    -     (iv) ANTI-SENSE: NO    -      (v) FRAGMENT TYPE: internal    -     (vi) ORIGINAL SOURCE:    -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:    #Glu Ser Arg Tyr Arg Leuyr Thr Tyr Lys Arg    #                 15    #Thr Pro Gly Arg Arg Meter Asp Ile Ile Asp    #             30    #Ser Asp Lys Val Ser Arger Ala Arg Leu Leu    #         45    #Glu Ser Trp Arg Met Metal His Ile Gly Asp    #     60    #Val Ile Gly Glu Glu Valer Val Pro Val Ser    # 80    #Lys Asn Ala Ile Asn Leurg Glu Asn Asp Ile    #                 95    -  Met Phe Trp Gly Ile                 100    - (2) INFORMATION FOR SEQ ID NO:5:    -      (i) SEQUENCE CHARACTERISTICS:    #acids    (A) LENGTH: 126 amino              (B) TYPE: amino acids              (C) STRANDEDNESS: single              (D) TOPOLOGY: linear    -     (ii) MOLECULE TYPE: protein    -    (iii) HYPOTHETICAL: NO    -     (iv) ANTI-SENSE: NO    -      (v) FRAGMENT TYPE: internal    -     (vi) ORIGINAL SOURCE:    -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:    #Pro Gly Asp Pro Leu Glusn Ser Ser Ser Val    #                 15    #Gln Phe Lys Val Tyr Thrla Ser Leu Ser Leu    #             30    #Val Asp Val Gln Ser Asprg Tyr Arg Leu Phe    #         45    #Ile Pro Leu Ala Ser Alaly Arg Arg Met Val    #     60    #Leu Tyr Pro Val Val Hisys Val Ser Arg Glu    # 80    #Thr Asp Met Ala Ser Valrp Arg Met Met Thr    #                 95    #Asp Leu Ser His Arg Gluly Glu Glu Val Ala    #            110    #Phe Trp Gly Ileys Asn Ala Ile Asn Leu Met    #        125    - (2) INFORMATION FOR SEQ ID NO:6:    -      (i) SEQUENCE CHARACTERISTICS:    #acids    (A) LENGTH: 126 amino              (B) TYPE: amino acids              (C) STRANDEDNESS: single              (D) TOPOLOGY: linear    -     (ii) MOLECULE TYPE: protein    -    (iii) HYPOTHETICAL: NO    -     (iv) ANTI-SENSE: NO    -      (v) FRAGMENT TYPE: internal    -     (vi) ORIGINAL SOURCE:    -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:    #Cys Arg Ser Thr Leu Gluro Ser Leu His Ala    #                 15    #Gln Phe Lys Val Tyr Threr Ser Asn Ser Leu    #             30    #Val Asp Val Gln Ser Asprg Tyr Arg Leu Phe    #         45    #Ile Pro Leu Ala Ser Alaly Arg Arg Met Val    #     60    #Leu Tyr Pro Val Val Hisys Val Ser Arg Glu    # 80    #Thr Asp Met Ala Ser Valrp Arg Met Met Thr    #                 95    #Asp Leu Ser His Arg Gluly Glu Glu Val Ala    #            110    #Phe Trp Gly Ileys Asn Ala Ile Asn Leu Met    #        125    __________________________________________________________________________

What is claimed is:
 1. An autonomously replicating cloning and/orsequencing vector adapted to transform a procaryotic cell, said vectorcomprising at least one promoter nucleotide sequence and at least onenucleotide sequence which encodes a fusion protein which is active as apoison to the procaryotic cell, said nucleotide sequence which encodes afusion protein being obtained by fusing a coding nucleotide sequence,which comprises several cloning sites, and a nucleotide sequence whichencodes protein poison CcdB.
 2. The vector according to claim 1, whereinsaid vector is a recombinant plasmid.
 3. The vector according to claim1, wherein the nucleotide sequence which encodes the protein poisonlacks the cleavage site for the restriction enzyme Smal.
 4. The vectoraccording to claim 1, wherein the vector corresponds to deposition No.LMBP2781.
 5. A procaryotic cell which is transformed with the cloningvector according to claim
 1. 6. A method for selecting recombinantclones, comprising:inserting an oligodeoxynucleotide into the codingnucleotide sequence of a vector according to claim 1; transforming aprocaryotic cell susceptible to the poison protein with the vector; andselecting recombinant clones which survive.
 7. An autonomouslyreplicating cloning and/or sequencing vector adapted to transform aprocaryotic cell, said vector being a recombinant virus and comprisingat least one promoter nucleotide sequence and at least one nucleotidesequence which encodes a fusion protein which is active as a poison tothe procaryotic cell, said nucleotide sequence which encodes a fusionprotein being obtained by fusing a coding nucleotide sequence, whichcomprises several cloning sites, and a nucleotide sequence which encodesprotein poison CcdB.
 8. An autonomously replicating cloning and/orsequencing vector adapted to transform a procaryotic cell, said vectorbeing a recombinant pUC plasmid and comprising at least one promoternucleotide sequence and at least one nucleotide sequence which encodes afusion protein which is active as a poison to the procaryotic cell, saidnucleotide sequence which encodes a fusion protein being obtained byfusing a coding nucleotide sequence, which comprises several cloningsites, and a nucleotide sequence which encodes protein poison CccdB. 9.An autonomously replicating cloning and/or sequencing vector adapted totransform a procaryotic cell, said vector comprising at least onerepressible promoter nucleotide sequence, which consists of the Lacoperon promoter, and at least one nucleotide sequence which encodes afusion protein which is active as a poison to the procaryotic cell, saidnucleotide sequence which encodes a fusion protein being obtained byfusing a coding nucleotide sequence, which comprises several cloningsites, and a nucleotide sequence which encodes protein poison CcdB. 10.An autonomously replicating cloning and/or sequencing vector adapted totransform a procaryotic cell, said vector comprising at least onepromoter nucleotide sequence and at least one nucleotide sequence whichencodes a fusion protein which is active as a poison to the procaryoticcell, said nucleotide sequence which encodes a fusion protein beingobtained by fusing a coding nucleotide sequence, which comprises severalcloning sites, and a nucleotide sequence which encodes protein poisonCcdB, wherein the cloning sites of the coding nucleotide sequence areabsent on the remainder of the nucleotide sequence of the cloningvector.
 11. A procaryotic host cell for a cloning vector which encodes afusion protein which is obtained by fusing a coding nucleotide sequence,which comprises several cloning sites, and a nucleotide sequence whichencodes protein poison CcdB, wherein the cell possesses a mutation whichconfers resistance to the poisonous activity of the fusion proteinand/or possesses a gene which encodes a protein which is an antipoisonto the fusion protein.
 12. The cell according to claim 11, which is anEscherichia coli cell, wherein the cell possesses a mutation in whicharginine 462 is replaced with a cysteine in the amino acid sequence ofthe GyrA polypeptide of gyrase.
 13. The cell according to claim 11,wherein the cell is that deposited under No. LMGP-12601.
 14. Theprocaryotic host cell of claim 11, wherein the cell possesses achromosomal I^(q) and an increased transformation efficiency.